Herbert Holt Park is the home of Gatlinburg's Trout Rearing Facility, which is Tennessee's only municipal trout farm. The facility is managed to maintain a trout fishery, specifically rainbow trout, within the city limits of Gatlinburg. Fish reared at the facility are transported, in loads ranging from 350 to 500 lbs. per stocking, and deposited into the West Prong of the Little Pigeon River every Thursday (no fishing is allowed on Thursday).

The waters around Herbert Holt Park, including a short distance upstream and downstream, are designated as "children only-" fishing. The park also provides a handicap-accessible fishing pier within this section of children only waters. The pier allows physically handicapped individuals to access the river to fish and is significant because it is the first fast-water handicap fishing pier in the State of Tennessee. The pier is restricted to children and physically handicapped persons of all ages. All other fishing regulations apply including a creel limit (only 2 fish may be kept while fishing this section of stream). All fish must be released between December 1 and March 31.

The West Prong of the Little Pigeon River is the main waterway that travels through Gatlinburg's city limits. It is accessible to fishermen from the north entrance to Gatlinburg on U.S. 441, through downtown and up to the Great Smoky Mountains National Park boundary at the southern end of Gatlinburg. There are three tributaries of the West Prong that are also included in Gatlinburg's fishable waters: Roaring Fork Creek, and LeConte Creek.

There is a second "children only" section of stream at Mynatt Park located along LeConte Creek. This section extends from the Great Smoky Mountains National Park Boundary, at the beginning of the Historic Motor Nature Trail, to approximately a quarter mile downstream from Mynatt Park.